Thesis Defense - Public Seminar: GENERATING IPSC DERIVED CELLS FOR FLAVIVIRUS-HOST INTERACTION STUDIES
Flavivirus, in particular Dengue virus, impose a significant burden on health and social systems. While great progress has been made in dissecting virus host interactions and understanding mechanisms that drive disease pathology, the majority of such studies employ cell lines. Unfortunately, cell lines are plagued with potentially confounding factors such as abnormal karyotypes, altered gene express, often attenuated immune responses and immortalization, making determining the underlying molecular mechanism responsible pathogenesis, particularly age-dependent disease outcome, particularly elusive. Here, senescent diploid cells WI-38 and MRC-5 were naturally immortalized by reprogramming them to iPSCs. These iPSCs provided a reservoir from which cells could be differentiated and undergo the natural process of senescence. This offered the opportunity to study are-related disease outcome in the context of flavivirus infection in terms of both host response and viral infection. Taken together, iPSC derived cells can be used to study the interplay between viral infection in the context of distinct host cell types at different replicative ages, providing a potentially powerful tools in scrutinizing age-dependent disease outcomes.
Prof. Ooi Eng Eong
875 9186 9978
Date and Time
28 Jul 2021 @ 09:00 - 28 Jul 2021 @ 10:00
IBM PhD PROGRAM (INTAKE 2016)