Sugii, Shigeki

PhD

Assistant Professor

Cardiovascular & Metabolic Disorders Programme

Other Affiliations:

Group Leader, Singapore Bioimaging Consortium, A*STAR

Adjunct Assistant Professor, NTU Lee Kong Chian School of Medicine

+65 6478 8763

+65 6478 9957

Email 1:

shigekisugii@gmail.com; shigeki_sugii@sbic.a-star.edu.sg

http://www.sugii.org/

Research Bio Container

Research

Research Interest:

The overall research theme of our group is to understand molecular identity and functions of fat-derived stem cells and to make use of them for treating metabolic disorders.

1. Molecular identification of adipose-derived stem cells
A research interest of our group is to delineate the molecular identity of adipose-derived stem cells (ASC) and metabolic pathways initiating adipocyte differentiation by using bioimaging and other analytic approaches. We are investigating several mouse and human models of obesity, lipodystrophy and other metabolic disorders. Active studies are also ongoing to study the biological difference between adipose stem cells from visceral and subcutaneous fat depots. For example, through high throughput image screening, we recently identified specific cell surface markers, CD10 for human subcutaneous ASC and CD200 for visceral ASC. We are also using other factors as a biological tool to track early adipose development; e.g. PPARgamma, which a ligand-regulated transcription factor and a master regulator of adipogenesis.

2. Therapeutic application of adipose-derived stem cells
Fat tissue is an abundant source of proliferating, multipotent stem cells, which offers high potentials for regenerative medicine. Our previous work demonstrated that the adipose-derived stem cells (ASC) both from human and mice are capable of reprogramming into induced pluripotent stem cells (iPSC) with high efficiencies and in the absence of feeder cells. It is now possible to use this ASC-iPSC system to engineer the cells into clinically useful cell types (e.g. cardiomyocytes, pancreatic beta cells, hepatocytes, brown adipocytes) for regenerative therapeutics and study development of metabolic pathways. By collaborating with other groups including imaging specialists and clinician scientists, we develop a suitable platform for cellular modeling, transplantation and tracking where human fat biopsies are converted into relevant cell types, enabling us to explore molecular mechanisms and treatment options for metabolic diseases. This ASC-iPSC system will be valuable for personalized drug screening/testing, human disease modeling, and ultimately, cell therapies.

Selected Publications:

Recent Publications

1. Dinish, U.S., Wong, C.L., Sriram, S., Ong, W.K., Balasundaram, G., Sugii, S.*, and Olivo, M.* (2017) Diffuse Optical Spectroscopy and Imaging to Detect and Quantify Adipose Tissue Browning. Sci. Rep. 7, 41357.

2. Perumal, J., Sriram, S., Lim, H.Q., Olivo, M.*, Sugii, S.* (2016) Retinoic Acid Is Abundantly Detected in Different Depots of Adipose Tissue by SERS. Adipocyte 5, 378-383.

3. Chaurasia, B., Kaddai, V.A., Lancaster, G.I., Henstridge, D.C., Sriram, S., Galam, D.L.A., Gopalan, V., Bhanu Prakash, K.N., Velan, S.S., Bulchand, S., Tsong, T.J., Wang, M., Siddique, M.M., Yuguang, G., Sigmundsson, K., Mellet, N.A., Weir, J.M., Meikle, P.J., Bin Mohamed Yassin, M.S., Shabbir, A., Shayman, J.A., Hirabayashi, Y., Toh, S.A., Sugii, S., and Summers, S.A. (2016) Adipocyte Ceramides Regulate Subcutaneous Adipose Browning, Inflammation and Metabolism. Cell Metab. 24, 820-834.

4. Takeda, K., Sriram, S., Chan, X.H.D., Ong, W.K., Yeo, C.R., Tan, B., Lee, S.A., Kong, K.V., Hoon, S., Jiang, H., Yuen, J.J., Perumal, J., Agrawal, M., Vaz, C., So, J., Shabbir, A., Blaner, W.S., Olivo, M., Han, W., Tanavde, V., Toh, S.A., and Sugii, S. (2016) Retinoic Acid Modulates Visceral-Specific Adipogenic Defects of Human Adipose-derived Stem Cells. Diabetes 65, 1164-1178.

5. Lysaght, T., and Sugii, S. (2016) Uncertain Oversight of Regenerative Medicines in Japan under the ASRM. Cell Stem Cell 18, 438-439.

6. Srijaya, T.C., Sriram, S., Sugii, S.*, and Abu Kasim, N.H.* (2016) Stem Cells in Dentistry: Potential Applications and Perspectives in Clinical Research. In P.V. Pham (Ed.), Stem Cells in the Clinical Applications, In Press, Springer (*Co-corresponding Author)

7. Park, M., Kaddai, V., Ching, J., Fridanto, K.T., Sieli R.J., Sugii, S., and Summers, S.A. (2016) A Role for Ceramides, but NOT Sphingomyelins, as Antagonists of Insulin Signaling and Mitochondrial Metabolism in C2C12 Myotubes. J. Biol. Chem. 291, 23978-23988.

8. Gunet, V., Tan, N.S., Sugii, S., Lim, T.C., Wong, T.C., Choong, C. (2016) Comparative Study of Adipose-Derived Stem Cells from Abdomen and Breast. Ann. Plast. Surg. 76, 569-575.

9. Bhanu Prakash, K.N., Verma, S.K., Yaligar, J., Goggi, J., Gopalan, V., Lee, S.S., Tian, X., Sugii, S., Leow, M.K., Bhakoo, K., and Velan, S.S. (2016) Segmentation and Characterization of Interscapular Brown Adipose Tissue in Rats by Multi-Parametric Magnetic Resonance Imaging. MAGMA 29, 277-286.

10. Ong, W.K., Tan, C.S., Chan, K.L., Goesantoso, G.G., Chan, X.H.D., Chan, E., Yin, J., Yeo, C.R., Khoo, C.M., So, J., Shabbir, A., Toh, S.A., Han, W., and Sugii, S. (2014) Identification of Specific Cell Surface Markers of Adipose-derived Stem Cells from Subcutaneous and Visceral Fat Depots. Stem Cell Reports 2, 171-179.

11. Lim, M.H., Ong, W.K., and Sugii, S. (2014) Current Landscape of Adipose-derived Stem Cells in Clinical Applications. Expert Rev. Mol. Med. 16, e8.

12. Raichur, S., Wang, S.T., Chan, P.W., Li, Y., Ching, J., Chaurasia, B., Dogra, S., Öhman, M.K., Takeda, K., Sugii, S., Pewzner-Jung, Y., Futerman, A.H., and Summers, S.A. (2014) CerS2 haploinsufficiency inhibits β-oxidation and confers susceptibility to diet-induced steatohepatitis and insulin resistance. Cell Metab. 20, 687-695.

13. Wee, K., Yang, W., Sugii, S., and Han, W. (2014) Towards a mechanistic understanding of lipodystrophy and seipin functions. Biosci. Rep. 34, e00141.

14. Yang, W., Thein, S., Lim, C.Y., Ericksen, R.E., Sugii, S., Xu, F., Robinson, R., Kim, J.B., and Han, W. (2014) Arp2/3 Complex Regulates Adipogenesis by Controlling Cortical Actin Remodeling. Biochem. J. 464, 179-92.

15. Choong, P.F., Teh, H.X., Teoh, H.K., Ong, H.K., Choo, K.B., Sugii, S., Cheong, S.K., Kamarul, T. (2014) Heterogeneity of Osteosarcoma Cell Lines Led to Variable Responses in Reprogramming. Int. J. Med. Sci. 11, 1154-1160.

16. Huang, C.J., Nguyen, P.N.N., Choo, K.B., Sugii, S., Wee, K., Cheong, S.K., Kamarul, T. (2014) Frequent Co-Expression of miRNA-5p and -3p Species and Cross-Targeting in Induced Pluripotent Stem Cells. Int. J. Med. Sci. 11, 824-833.

17. Yaligar, J., Gopalan, V., Ong, W.K., Sugii, S., Shui, G., Lam, B.D.; Henry, C.J., Wenk, M.R., Tai, E.S., and Velan, S.S. (2014) Evaluation of Dietary Effects on Hepatic Lipids in High Fat and Placebo Diet Fed Rats by In Vivo MRS and LC-MS Techniques. PLoS ONE. 9, e91436.

18. Gong, Y.D., Zhang, B., Notake, T., Minamide, H., Olivo, M., and Sugii, S. (2014) Investigations on Polarimetric Terahertz Frequency Domain Spectroscopy. Appl. Phys. A. 115, 83-86.

19. Ong, W.K., and Sugii, S. (2013) Adipose-derived Stem Cells: Fatty Potentials for Therapy. Int. J. Biochem. Cell Biol. [“Cells in Focus” series] 45, 1083-1086.

20. Qiu, W., Wee, K., Takeda, K., Lim, X., Sugii, S.*, Radda, G.K., and Han, W*. (2013) Suppression of Adipogenesis by Pathogenic Seipin Mutant Is Associated with Inflammatory Response. PLoS ONE 8, e57874.

21. Yang, W., Thein, S., Guo, X., Xu, F., Venkatesh, B., Sugii, S., Radda, G.K., and Han, W. (2013) Seipin Differentially Regulates Lipogenesis and Adipogenesis through a Conserved Core Sequence and an Evolutionarily Acquired C-terminus. Biochem. J. 452, 37-44.

22. Yang, W., Guo, X., Thein, S., Xu, F., Sugii, S., Baas, P.W., Radda, G.K., and Han, W. (2013) Regulation of Adipogenesis by Cytoskeleton Remodeling Is Facilitated by Acetyltransferase MEC-17-Dependent Acetylation of α-Tubulin. Biochem. J. 449, 605-612.

23. Sugii, S., and Evans, R.M. (2011) Epigenetic Codes of PPARγ in Metabolic Disease. FEBS Lett. [REVIEW ARTICLE] 585, 2121-2128.

24. Sugii, S., Kida, Y., Berggren, W.T., and Evans, R.M. (2011) Feeder-Dependent and Feeder-Independent iPS Cell Derivation from Human and Mouse Adipose Stem Cells. Nature Protoc. 6, 346-358.

25. Sugii, S., Kida, Y., Kawamura, T., Suzuki, J., Vassena, R., Yin, Y.Q., Lutz, M.K., Berggren, W.T., Izpisua Belmonte, J.C., and Evans, R.M. (2010) Human and Mouse Adipose-Derived Cells Support Feeder Independent Induction of Pluripotent Stem Cells. Proc. Natl. Acad. Sci. USA 107, 3558-3563.

*Co-corresponding Author

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