THESIS DEFENSE — PUBLIC SEMINAR: HSP83/HSP90 PROMOTES DROSOPHILA NEURAL STEM CELL REACTIVATION THROUGH PHYSICAL ASSOCIATION WITH INR

Start Date & Time: 
Friday, 25 January, 2019 - 10:00
End Date & Time: 
Friday, 25 January, 2019 - 11:00
Venue: 

Amphitheatre, Level 2, Duke-NUS

Speaker: 
Speaker Details: 

HUANG JIAWEN
IBM PhD PROGRAM (INTAKE 2014)

Synopsis: 

In the mammalian adult brain, the majority of adult neural stem cells (NSCs) are quiescent. Interestingly, these quiescent NSCs can be reactivated in response to stimuli. In the Drosophila larval brain, the insulin receptor (InR)/PI3K/Akt pathway (i.e. insulin pathway) triggers the reactivation of the NSCs. However, the mechanisms that control the insulin pathway during reactivation are largely unknown. Here, I have identified Heat shock protein 83 (Hsp83/Hsp90), a molecular chaperone, as a novel intrinsic regulator of NSC reactivation. Hsp83 is both necessary and sufficient for NSC reactivation by promoting the activation of InR pathway in larval brains in the presence of dietary amino acids. Both Hsp83 and its co-chaperone Cdc37 physically interact with InR. Remarkably, hsp83 knockdown phenotype is rescued by over-activating the insulin pathway. This study reveals that Hsp83 promotes NSC reactivation via interacting with InR of the insulin pathway. Both Hsp83 and the pathways governing NSC reactivation in Drosophila are highly conserved in mammalian systems. My results will not only broaden the understanding of NSC reactivation regulation in Drosophila, but also provide insights into understanding the homeostasis of mammalian NSCs.

Thesis Advisor: A/Prof. Wang Hongyan