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NBD Virtual Seminar Series: Brillouin microscopy
Abstract:Brillouin imaging can extract viscoelastic properties with micron-level resolution in a label-free, non- invasive way. The conventional bulk-optics based Brillouin system has already been applied to various biological samples. While the utility of Brillouin imaging has been demonstrated, the intrinsically weak scattering process means that recent work in instrumentation has aimed to maximize the efficiency of existing technology, as well as making it more compact and portable. For more systematic optimization strategies, the theoretical parameters to evaluate the performance of arbitrary Brillouin spectrometers have been proposed for the first time. This enables the optimization of Brillouin systems in general. In this talk he will present the latest developments in Brillouin instrumentation. This also includes software-based reconstruction techniques to enhance the SNR of the system. These methods are more attractive for their wider applicability and have been found to be capable of extracting useful Brillouin shift value with low SNR in simulation and experiment.
Recently, the meaning of Brillouin measurements and its correlation to stiffness has been further investigated. It has been shown that the influence of water content in the mechanical behaviour of hydrated samples may dominate the Brillouin shift value. The addition of a Raman mode to measure this relative change in hydration may help to yield more accurate mechanical measurements. The correlative study of hydrogels was thus demonstrated as to show that inelastic spectroscopy in tandem is viable.
The talk will conclude by a summary other work being carried out in his group including instrument building capabilities.
Join Zoom Meeting
https://nus-sg.zoom.us/j/81545133269?pwd=NC8rVmxtajg1QmNaWllydytBWmg5UT09
Meeting ID: 815 4513 3269
Passcode: 161963
Host:
Assoc Prof Hyunsoo Shawn Je
Principal Investigator
Neuroscience & Behavioural Disorders Programme
Duke-NUS
Contact Person:
Joyceline Ng (joyceline.ng@duke-nus.edu.sg)
Neuroscience & Behavioural Disorders Programme
Duke-NUS
All are welcome. No registration is required.
Date and Time
18 Feb 2022 @ 11:00 - 18 Feb 2022 @ 12:00
Speaker
Professor Peter Török
Professor of Optical Physics
SCELSE Director, Imaging
Peter has rich experience in designing and building precision optical system also including instruments that have been commercially sold to world leading companies. He spent most of his working life in optical and confocal microscopy, polarised light imaging, optical data storage, electromagnetic imaging theory, compressive imaging, information theoretic aspects of imaging and spectroscopic imaging, including Raman and Brillouin modalities.