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Bi Xuezhi

Adjunct Associate Professor


Dr. Bi Xuezhi obtained his PhD in 2000 from Wuhan University, China, followed by postdoctoral training at the International Rice Research Institute and National University of Singapore. He joined A*STAR as a research scientist and mass spectrometry facility manager in 2009, where he contributed to chemical proteomics for chemical probe/drug target identification, protein PTM and interacting partners characterization. Later, he was appointed as of the head of proteomics group at Bioprocessing Technology Institute, focusing on mass spectrometry based biotherapeutics product quality attribute characterization. His work also involves proteomic profiling of upstream and downstream CHO culture processes, working closely with biotechnology and biopharmaceutical companies.  All his research subjects are related to protein and proteomics, ranging from rice, dust mites, fungus, virus, tears, sweat, cancer cell lines and tissue, embryonic stem cells, platypus venom to CHO cell-produced biotherapeutics. With many research collaborations with faculty members from signature programmes such as Cancer & Stem Cell Biology, and Cardiovascular & Metabolic Disorders, he joined the faculty as an Adjunct Associate Professor, Office of Research at Duke-NUS in 2019 to share his proteomics expertise.

My research interests focuses on the characterization of protein targets of pharmaceuticals, protein-protein interaction partners, protein posttranslational modifications (PTMs), mechanistic understanding of metabolomics (enzymes) pathway/ signal transduction networks involved in human cancer and stem cell development and cardiovascular metabolic disease regulations using high resolution mass spectrometry (HRMS) chemical proteomics and next generation of chemical labeled or label free quantitative proteomics strategies. I am also interested in developing new methods for deep proteome profiling and quantitative analysis of new PTM characterization such as challenging protein lipid modifications, O-glycosylation, methylation, ubiquitination, acetylation and phosphorylation of proteins.

My current research focuses on novel mass spectrometry technologies for characterization of biotherapeutic proteins and new modalities (critical quality attributes and biopharmaceuticals impurity analysis) along with their  biomanufacturing processes.

Another area of focus is the application of DIA-SWATH-MS in disease biomarker discovery, drug development, biopharmaceuticals manufacturing, food and nutrition processing related allergen and disease related pathogen characterization.

Sim, K.H., Liu, L.C.Y.,  Tan, H.T., Tan, K., Ng, D., Zhang, W., Yang, Y., Tate, S, Bi, X*.  A comprehensive CHO SWATH-MS spectral library for robust quantitative profiling of 10,000 proteins, Sci Data, DOI:10.1038/s41597-020-00594-z (2020)

Hu, Z., Tan, D.E.K., Chia, G., Tan, H., Leong, H.F., Chen, B.J., Lau, M.S., Tan, K.Y.S., Bi, X., Yang, D., Ho, Y.S.,  Wu, B., Bao, S., Wong, E.S.M., Tee, W.W. Maternal factor NELFA drives a 2C-like state in mouse embryonic stem cells. Nat Cell Biol, 1-12 (2020)

Ong, ST, Ng, A.S., Ng, X.R., Zhuang,Z., Wong, B.H.S., Prasannan,P., Kok,Y.J., Bi, X.,  Shim,H.,  Wulff, H., Chandy, K. G., Verma, N.K. Extracellular K+ Dampens T Cell Functions: Implications for Immune Suppression in the Tumor Microenvironment.  Bioelectricity, 1(3): 169-179 (2019)

Bi, X*., Ye, L., Lau, A., Kok, Y.J., Zheng, L., Ng, D., Tan, K., Ow, D., Ananta, E., Vafiadi, C. and Muller, J. Proteomic profiling of barley spent grains guides enzymatic solubilization of the remaining proteins. Appl Microbiol Biotechnol 102, 4159-4170 (2018) 

Yan,T., Ooi,W.F., Qamra, A., Cheung, A., Ma,D., Sundaram,G.M., Xu,C., Manjie Xing,M.,  Poon,L.F., Ho, J H.J., Ramlee,M.K., Wang,J., Aswad,L., Rozen, S.G., Ghosh,S., Bard, F.A., Sampath,P., Tergaonkar,V., Goh,E., Bi, X.  Fullwood, M.J., Tan, P., and  Li, S. HoxC5 and miR-615-3p target newly evolved genomic regions to repress hTERT and inhibit tumorigenesis, Nature Commun, 9:100 (2018).

Alagu, J., Itahana, Y., Sim, F., Chao, S.H., Bi, X. and Itahana, K. Tumor Suppressor p14ARF Enhances IFN-gamma-Activated Immune Response by Inhibiting PIAS1 via SUMOylation. J Immunol 201, 451-464 (2018)

Hutchinson, D., Muller, J., McCarthy, J.E., Gun'ko, Y.K., Verma, N.K., Bi, X., Di Cristo, L., Kickham, L., Movia, D., Prina-Mello, A. and Volkov, Y. Cadmium nanoparticles citrullinate cytokeratins within lung epithelial cells: cadmium as a potential cause of citrullination in chronic obstructive pulmonary disease. Int J Chron Obstruct Pulmon Dis 13, 441-449. (2018)

Neo, S.H., Lew, Q.J., Koh, S.M., Zheng, L., Bi, X., and Chao, S.H. Use of a novel cytotoxic HEXIM1 peptide in the directed breast cancer therapy. Oncotargets, 7(5):5483-94 (2016)

Lim, C.Y., Bi, X., Wu D., Kim J.B., Gunning, P.W., Hong, W., and Han, W. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodelling. Nature Commun,  6:5951 (2015)

Lee, K.G., Kim, S.S., Kui, L., Voon, D.C., Mauduit, M., Bist, P., Bi, X., Pereira, N.A., Liu, C., Sukumaran, B., Liu, C., Sukumaran, B., Rénia, L., Ito,Y.,  Lam, K.P. Bruton's tyrosine kinase phosphorylates DDX41 and activates its binding of dsDNA and STING to initiate type 1 interferon response. Cell Rep 10, 1055-1065 (2015)

Yun, S. W., Leong, C., Bi, X., Ha, H.H., Yu, Y. H., Tan, Y. L., Narayanan, G., Sankaran, S., Kim, J. Y., Hariharan, S., et al. A fluorescent probe for imaging symmetric and asymmetric cell division in neurosphere formation. Chem Commun, 50: 7492-7494 (2014)

Maury, J.J., Ng, D., Bi, X., Bardor, M., and Choo, A.B. (2014) Multiple reaction monitoring mass spectrometry for the discovery and quantification of O-GlcNAc-modified proteins. Anal Chem 86, 395-402 

Yun, S. W., Leong, C., Zhai, D., Tan Y. L., Lim, L., Bi, X., Lee, J. J., Kim, H. J., Kang, N.Y., Ng S.H., Stanton, L.W., and Chang, Y.T. Neural stem cell specific fluorescent chemical probe binding to Fabp7. Proc Natl Acad Sci USA, 109: 10214-10217. (2012)

Kim, Y. K., Lee, J.-S., Bi, X., Ha, H.-H., Ng, S.H., Ahn, Y.-h., Lee, J.-J., Wagner, B.K., Clemons, P. A. and Chang, Y.T. The binding of fluorophores to proteins depends on the cellular environment. Angew. Chem. Int., 50: 2761–2763 (2011)

Bi, X., Lin, Q., Foo, T. W., Joshi, S., You, T., Shen, H.M., Ong, C.N., Cheah, P. ., Eu, K. W., and Hew, C.L. Proteomic analysis of colorectal cancer reveals alterations in metabolic pathways: mechanism of tumorigenesis. Mol Cell Proteomics, 5, 1119-1130 (2006)

Bi, X.*, Khush, G.S., and Bennett, J. The rice nucellin orthologue, OsAsp1, which encodes an active aspartic protease without plant-specific insert, is strongly expressed in early embryo. Plant and Cell Physiology. 46:87-98 (2005) (*corresponding author)