AMPAR Intracellular Transport and Synaptic Physiology

Start Date & Time: 
Thursday, 12 January, 2017 - 15:00
End Date & Time: 
Thursday, 12 January, 2017 - 16:00
Venue: 

Duke-NUS Medical School

Room 7C (Level 7)

Speaker: 
Speaker Details: 

Dr Francoise Coussen
Research Director
CNRS, France

Synopsis: 

AMPARs mediate fast excitatory synaptic transmission in the central nervous system. Their abundance at the synapse is essential for the establishment and maintenance of synaptic function. Their synaptic localization is dependent on dynamic exocytosis, endocytosis and plasma membrane trafficking events. Our hypothesis is that localization of AMPARs is also regulated by their intracellular transport. However, AMPARs post-ER trafficking toward the plasma membrane is still poorly understood. Using a new biochemical tool combined with live imaging, we studied the dynamic secretion AMPARs in hippocampal neurons. We characterized AMPAR trafficking from the ER to the Golgi and from Golgi to the plasma membrane. We analyzed the characteristics of basal AMPAR transport and studied the influence of synaptic plasticity in the transport AMPARs. SAP97 has been shown to be involved in the intracellular AMPARs traffic via its PDZ interaction with GluA1, we studied its role in the GluA1 vesicular trafficking. We are characterizing how interaction of GluA1 with SAP97 can regulate the intracellular transport of AMPARs.

Biography: 

Dr Coussen obtained her PhD in 1986 at the Pasteur Institute as a biochemist. Post-doc in Gilman’s lab (1987-1988), with Pat Casey. Research officer CNRS 1988. Sabbatical at Duke University with H Erickson (1994-1996). Bordeaux as a director of research in C Mulle lab (1997-2012) and in D Choquet (2012-2016) in the Institute for Interdisciplinary Neuroscience. She combines neuroscience, imaging technology and biochemistry to study the transport and the molecular complex associated with AMPARs.

Host: 

Prof Patrick Casey
Senior Vice Dean of Research
Duke-NUS Medical School

Contact Person: 

Ms Kathleen Chan, Duke-NUS Research Affairs

E-mail : Kathleen.chan@duke-nus.edu.sg