Dendritic Cells: Matching Mice and Men

Start Date & Time: 
Tuesday, 2 April, 2013 - 15:00
End Date & Time: 
Tuesday, 2 April, 2013 - 16:00
Venue: 

Duke-NUS, Amphitheatre, 2nd Floor

Speaker: 
Speaker Details: 

Principal Investigator, Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR)

Synopsis: 

Dendritic cells (DCs) are heterogeneous immune cells crucial for both defense against pathogens and tolerance.  DC populations in mouse and human non-lymphoid tissues can be separated into functionally different subsets, that include human CD141high DCs, CD1c+ DCs and CD14+ DCs and murine CD103+ DCs and CD11b+ DCs. We recently showed that human CD141high DCs share common gene expression and functionality with mouse tissue CD103+ DCs such as their cross-presentation capacity (Haniffa, Immunity 2012).  However, the precise functional interspecies correlation of the remaining subsets remains incompletely defined. Here, we show that murine lung and gut lamina propria CD11b+ DC populations are comprised of two ontogenically and functionally distinct cell types, bona fide Flt3- and IRF4-dependent CD24+CD64- DCs and contaminating CSF-1R-dependent CD24-CD64+ interstitial macrophages.Extending these findings to humans, we find that CD1c+ DCs are homologues of murine CD24+CD11b+ DCs, expressing IRF4 and promoting Th17 responses through IL23α secretion. Our data reveal heterogeneity in mouse CD11b+ DC compartment and identify in mucosal tissues an IRF4-expressing DC lineage specialized to instruct IL-17 responses in both mouse and human. The functional alignment of human and mouse DC subsets is essential for translating mouse in vivo findings to human primary DC immunobiology.

Biography: 

Florent Ginhoux graduated in Biochemistry from the University Pierre et Marie CURIE, Paris VI and obtained a Masters degree in Advanced Studies in Immunology from the Pasteur Institute, Paris.

Dr Ginhoux  then started his PhD in the Immunology Team of GENETHON, Evry and obtained his PhD in 2004 from the University Pierre et Marie CURIE, Paris VI. As a postdoctoral fellow, Florent Ginhoux joined the Laboratory of Miriam Merad in the Mount Sinai School of Medicine (MSSM), New York where he studied the ontogeny and the homeostasis of dendritic cell populations.

In 2008 he became an Assistant Professor in the Department of Gene and Cell Medicine, MSSM and member of the Immunology Institute of MSSM. He joined SIgN in March 2009 to lead a laboratory focusing on the ontogeny and function of dendritic cells and macrophages.

Host: 

Dr Manoj Krishnan

Assistant Professor 

Program in Emerging Infectious Diseases

Contact Person: 

Ms Serene Chee

Email: serene.chee@duke-nus.edu.sg